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Selective Enrichment and Fractionation of Phosphopeptides from Peptide Mixtures by Isoelectric Focusing after Methyl Esterification
We have developed a new strategy to enrich and fractionate phosphopeptides from peptide mixtures based on difference in their isoelectric points (pIs) after methyl esterification. After isoelectric focusing (IEF) of a methylated tryptic digest of a mixture of α-S-casein and β-casein, phosphopeptides...
Bewaard in:
| Hoofdauteurs: | , , , , |
|---|---|
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
2007
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2526126/ https://ncbi.nlm.nih.gov/pubmed/17249638 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac061606u |
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