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Peptide separations by on-line MudPIT compared to isoelectric focusing in an off-gel format: Application to a membrane-enriched fraction from C2C12 mouse skeletal muscle cells

High resolution peptide separation is pivotal for successful shot-gun proteomics. The need for capable techniques propels invention and improvement of ever more sophisticated approaches. Recently, Agilent Technologies has introduced the OFFGEL fractionator, which conducts peptide separation by isoel...

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Détails bibliographiques
Auteurs principaux: Elschenbroich, Sarah, Ignatchenko, Vladimir, Sharma, Parveen, Schmitt-Ulms, Gerold, Gramolini, Anthony O., Kislinger, Thomas
Format: Artigo
Langue:Inglês
Publié: 2009
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3712977/
https://ncbi.nlm.nih.gov/pubmed/19670906
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr900318k
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