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Improved Enrichment Strategies for Phosphorylated Peptides on Titanium Dioxide Using Methyl Esterification and pH Gradient Elution
Improvements to phosphopeptide enrichment protocols employing titanium dioxide (TiO(2)) are described and applied to identification of phosphorylation sites on recombinant human cyclin-dependent kinase 2 (CDK2). Titanium dioxide binds phosphopeptides under acidic conditions and they can be eluted un...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2475647/ https://ncbi.nlm.nih.gov/pubmed/18396144 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.03.024 |
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