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Capillary isoelectric focusing after sample enrichment with immunoaffinity chromatography in a single capillary

For accurate micro-scale quantification of a specific protein in biological fluids, immunoaffinity chromatography (IAC) and isoelectric focusing (IEF) were combined in a single fused-silica capillary. The inner wall of the capillary was coated with an anti-E-tag antibody at the inlet side to form an...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Shimura, Kiyohito, Nagai, Toshihiko
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5156949/
https://ncbi.nlm.nih.gov/pubmed/27976690
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep39221
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