Single-Cell FRET Imaging of Transferrin Receptor Trafficking Dynamics by Sfp-Catalyzed, Site-Specific Protein Labeling

SUMMARY: Fluorescence imaging of living cells depends on an efficient and specific method for labeling the target cellular protein with fluorophores. Here we show that Sfp phosphopantetheinyl transferase-catalyzed protein labeling is suitable for fluorescence imaging of membrane proteins that spend...

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Autores principales: Yin, Jun, Lin, Alison J., Buckett, Peter D., Wessling-Resnick, Marianne, Golan, David E., Walsh, Christopher T.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2005
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2494879/
https://ncbi.nlm.nih.gov/pubmed/16183024
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2005.07.006
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