Purification and characterization of poliovirus polypeptide 3CD, a proteinase and a precursor for RNA polymerase.
A cDNA clone encoding the 3CD proteinase (3CDpro) of poliovirus type 2 (Sabin), the precursor to proteinase 3Cpro and RNA polymerase 3Dpol, was expressed in bacteria by using a T7 expression system. Site-specific mutagenesis of the 3C/3D cleavage site was performed to generate active proteolytic pre...
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| 出版年: | J Virol |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology (ASM)
1992
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC240456/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1331532/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.66.12.7481-7489.1992 |
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