Combinatorial engineering to enhance thermostability of amylosucrase

Amylosucrase is a transglucosidase that catalyzes amylose-like polymer synthesis from sucrose substrate. About 60,000 amylosucrase variants from two libraries generated by the MutaGen random mutagenesis method were submitted to an in vivo selection procedure leading to the isolation of more than 700...

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Main Authors: Emond, Stéphane, André, Isabelle, Jaziri, Kais, Potocki-Véronèse, Gabrielle, Mondon, Philippe, Bouayadi, Khalil, Kharrat, Hakim, Monsan, Pierre, Remaud-Simeon, Magali
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2008
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2386748/
https://ncbi.nlm.nih.gov/pubmed/18441231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.083492608
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