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Novel product specificity toward erlose and panose exhibited by multisite engineered mutants of amylosucrase

A computer‐aided engineering approach recently enabled to deeply reshape the active site of N. polysaccharea amylosucrase for recognition of non‐natural acceptor substrates. Libraries of variants were constructed and screened on sucrose allowing the identification of 17 mutants able to synthesize mo...

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Dettagli Bibliografici
Pubblicato in:Protein Sci
Autori principali: Vergès, Alizée, Cambon, Emmanuelle, Barbe, Sophie, Moulis, Claire, Remaud‐Siméon, Magali, André, Isabelle
Natura: Artigo
Lingua:Inglês
Pubblicazione: John Wiley and Sons Inc. 2017
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5326559/
https://ncbi.nlm.nih.gov/pubmed/28019698
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3106
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