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Novel product specificity toward erlose and panose exhibited by multisite engineered mutants of amylosucrase
A computer‐aided engineering approach recently enabled to deeply reshape the active site of N. polysaccharea amylosucrase for recognition of non‐natural acceptor substrates. Libraries of variants were constructed and screened on sucrose allowing the identification of 17 mutants able to synthesize mo...
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| Publicado no: | Protein Sci |
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| Principais autores: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5326559/ https://ncbi.nlm.nih.gov/pubmed/28019698 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3106 |
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