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New Efficient Recombinant Expression System To Engineer Candida antarctica Lipase B
Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displa...
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| Autori principali: | , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
2010
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2849202/ https://ncbi.nlm.nih.gov/pubmed/20173074 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03057-09 |
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