Streamlined Preparation of Immobilized Candida antarctica Lipase B

[Image: see text] Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L(–1)) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catal...

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Bibliographic Details
Published in:ACS Omega
Main Authors: Cassimjee, Karim Engelmark, Hendil-Forssell, Peter, Volkov, Alexey, Krog, Anne, Malmo, Jostein, Aune, Trond Erik V., Knecht, Wolfgang, Miskelly, Iain R., Moody, Thomas S., Svedendahl Humble, Maria
Format: Artigo
Language:Inglês
Published: American Chemical Society 2017
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6045393/
https://ncbi.nlm.nih.gov/pubmed/30023589
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.7b01510
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