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Crystal structural analysis of protein–protein interactions drastically destabilized by a single mutation

The complex of barnase (bn) and barstar (bs), which has been widely studied as a model for quantitative analysis of protein–protein interactions, is significantly destabilized by a single mutation, namely, bs Asp39 → Ala, which corresponds to a change of 7.7 kcal·mol(−1) in the free energy of bindin...

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Detalhes bibliográficos
Main Authors: Urakubo, Yoshiaki, Ikura, Teikichi, Ito, Nobutoshi
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2386745/
https://ncbi.nlm.nih.gov/pubmed/18441234
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.073322508
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