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Crystallization and preliminary crystallographic analysis of an esterase with a novel domain from the hyperthermophile Thermotoga maritima

A predicted esterase (EstA) with an unusual new domain from the hyperthermophilic bacterium Thermotoga maritima has been cloned and overexpressed in Escherichia coli. The purified protein was crystallized by the hanging-drop vapour-diffusion technique in the presence of lithium sulfate and polyethyl...

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Detalhes bibliográficos
Main Authors: Sun, Lei, Levisson, Mark, Hendriks, Sjon, Akveld, Twan, Kengen, Servé W. M., Dijkstra, Bauke W., van der Oost, John
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2376313/
https://ncbi.nlm.nih.gov/pubmed/17768353
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910703953X
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