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Purification, crystallization and preliminary crystallographic analysis of a thermostable endonuclease IV from Thermotoga maritima

The DNA-repair enzyme endonuclease IV from the thermophilic bacterium Thermotoga maritima MSB8 (reference sequence NC_000853) has been expressed in Escherichia coli and crystallized for X-ray analysis. T. maritima endonuclease IV is a 287-amino-acid protein with 32% sequence identity to E. coli endo...

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Detalhes bibliográficos
Main Authors: Hughes, Ronny C., Tomanicek, Stephen J., Ng, Joseph D., Coates, Leighton
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2802891/
https://ncbi.nlm.nih.gov/pubmed/20054139
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109047393
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