Purification, crystallization and preliminary X-ray analysis of inositol dehydrogenase (IDH) from Bacillus subtilis

Inositol dehydrogenase (IDH) is an enzyme that catalyses the NAD(+)-dependent oxidation of myo-inositol to scyllo-inosose. The enzyme has been purified to homogeneity by means of Ni(2+)-affinity chromatography and was crystallized in both native and selenomethionine (SeMet) labelled forms using the...

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Bibliografiske detaljer
Main Authors: Van Straaten, K. E., Hoffort, A., Palmer, D. R. J., Sanders, D. A. R.
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2008
Fag:
Crystallization Communications
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374185/
https://ncbi.nlm.nih.gov/pubmed/18259059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108000328
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