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Expression, purification, crystallization and preliminary X-ray crystallographic analysis of l-­lactate dehydrogenase and its H171C mutant from Bacillus subtilis

l-Lactate dehydrogenase (LDH) is an important enzyme involved in the last step of glycolysis that catalyzes the reversible conversion of pyruvate to l-lactate with the simultaneous oxidation of NADH to NAD(+). In this study, wild-type LDH from Bacillus subtilis (BsLDH-WT) and the H171C mutant (BsLDH...

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Bibliografische gegevens
Hoofdauteurs: Zhang, Yanfeng, Gao, Xiaoli
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: International Union of Crystallography 2011
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3253837/
https://ncbi.nlm.nih.gov/pubmed/22232174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111048111
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