Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of YvoA from Bacillus subtilis

The putative transcriptional regulator protein YvoA (BSU35030) from Bacillus subtilis was cloned and heterologously expressed in Escherichia coli. The protein was purified by immobilized metal-affinity chromatography and size-exclusion chromatography and subsequently crystallized. A complete native...

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Bibliographic Details
Main Authors: Resch, Marcus, Roth, Heide Marie, Kottmair, Mathias, Sevvana, Madhumati, Bertram, Ralph, Titgemeyer, Fritz, Muller, Yves A.
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2009
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2664774/
https://ncbi.nlm.nih.gov/pubmed/19342794
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109008628
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