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Molecular cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of a purine nucleoside phosphorylase from Bacillus subtilis strain 168

Purine nucleoside phosphorylase (PNP; EC 2.4.2.1) is a key enzyme of the purine-salvage pathway. Its ability to transfer glycosyl residues to acceptor bases is of great biotechnological interest owing to its potential application in the synthesis of nucleoside analogues used in the treatment of anti...

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Detalhes bibliográficos
Main Authors: Martins, Nadia Helena, Meza, Andreia Navarro, Santos, Camila Ramos, de Giuseppe, Priscila Oliveira, Murakami, Mario Tyago
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3087654/
https://ncbi.nlm.nih.gov/pubmed/21543875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111010414
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