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Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168
The AmyX gene encoding pullulanase from the common spore-forming bacterium Bacillus subtilis strain 168 was cloned, overexpressed in Escherichia coli, purified and crystallized. The recombinant pullulanase was purified to homogeneity using ammonium sulfate precipitation, hydrophobic chromatography a...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2222569/ https://ncbi.nlm.nih.gov/pubmed/16582490 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106007901 |
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