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Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168

The AmyX gene encoding pullulanase from the common spore-forming bacterium Bacillus subtilis strain 168 was cloned, overexpressed in Escherichia coli, purified and crystallized. The recombinant pullulanase was purified to homogeneity using ammonium sulfate precipitation, hydrophobic chromatography a...

תיאור מלא

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מידע ביבליוגרפי
Main Authors: Malle, Dominggus, Itoh, Takafumi, Hashimoto, Wataru, Murata, Kousaku, Utsumi, Shigeru, Mikami, Bunzo
פורמט: Artigo
שפה:Inglês
יצא לאור: International Union of Crystallography 2006
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2222569/
https://ncbi.nlm.nih.gov/pubmed/16582490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106007901
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