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Purification, crystallization and preliminary X-ray analysis of NtdA, a putative pyridoxal phosphate-dependent aminotransferase from Bacillus subtilis
NtdA is a putative sugar aminotransferase that is required for the synthesis of 3,3′-neotrehalosadiamine. The enzyme was purified to homogeneity by means of Ni(2+)-affinity chromatography and was crystallized using the microbatch method. X-ray diffraction data were collected from a single crystal to...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2664778/ https://ncbi.nlm.nih.gov/pubmed/19342798 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109009038 |
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