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Purification, crystallization and preliminary X-ray analysis of NtdA, a putative pyridoxal phosphate-dependent aminotransferase from Bacillus subtilis

NtdA is a putative sugar aminotransferase that is required for the synthesis of 3,3′-neotrehalosadiamine. The enzyme was purified to homogeneity by means of Ni(2+)-affinity chromatography and was crystallized using the microbatch method. X-ray diffraction data were collected from a single crystal to...

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Main Authors: van Straaten, K. E., Langill, D. M., Palmer, D. R. J., Sanders, D. A. R.
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2664778/
https://ncbi.nlm.nih.gov/pubmed/19342798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109009038
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