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The effect of net charge on the solubility, activity, and stability of ribonuclease Sa

The net charge and isoelectric pH (pI) of a protein depend on the content of ionizable groups and their pK values. Ribonuclease Sa (RNase Sa) is an acidic protein with a pI = 3.5 that contains no Lys residues. By replacing Asp and Glu residues on the surface of RNase Sa with Lys residues, we have cr...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Shaw, Kevin L., Grimsley, Gerald R., Yakovlev, Gennady I., Makarov, Alexander A., Pace, C. Nick
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Cold Spring Harbor Laboratory Press 2001
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374010/
https://ncbi.nlm.nih.gov/pubmed/11369859
Tagiau: Ychwanegu Tag
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