Crystallization and preliminary crystallographic analysis of the catalytic domain of human flap endonuclease 1 in complex with a nicked DNA product: use of a DPCS kit for efficient protein–DNA complex crystallization

Flap endonuclease 1 (FEN1) is a structure-specific nuclease that removes the RNA/DNA primer associated with Okazaki fragments in DNA replication. Here, crystals of the complex between the catalytic domain of human FEN1 and a DNA product have been obtained. For efficient crystallization screening, a...

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Bibliographic Details
Main Authors: Sakurai, Shigeru, Kitano, Ken, Morioka, Hiroshi, Hakoshima, Toshio
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2007
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373994/
https://ncbi.nlm.nih.gov/pubmed/18097100
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107065372
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