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Expression, purification, crystallization and preliminary crystallographic analysis of human Rad GTPase

Human Rad is a new member of the Ras GTPase superfamily and is overexpressed in human skeletal muscle of individuals with type II diabetes. The GTPase core domain was overexpressed in Escherichia coli and purified for crystallization. Crystals were obtained at 293 K by vapour diffusion using a cryst...

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Detalhes bibliográficos
Main Authors: Yanuar, Arry, Sakurai, Shigeru, Kitano, Ken, Hakoshima, Toshio
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1978125/
https://ncbi.nlm.nih.gov/pubmed/16511212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105031982
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