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Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis
During protein integration into the endoplasmic reticulum, the N-terminal domain preceding the type I signal-anchor sequence is translocated through a translocon. By fusing a streptavidin-binding peptide tag to the N terminus, we created integration intermediates of multispanning membrane proteins....
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The Rockefeller University Press
2007
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2373506/ https://ncbi.nlm.nih.gov/pubmed/18166653 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.200707050 |
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