Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect

Short interfering RNA (siRNA)-based RNA interference (RNAi) is widely used for target gene knockdown in mammalian cells. To clarify the position-dependent functions of ribonucleotides in siRNA, siRNAs with various DNA substitutions were constructed. The following could be simultaneously replaced wit...

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Detalhes bibliográficos
Main Authors: Ui-Tei, Kumiko, Naito, Yuki, Zenno, Shuhei, Nishi, Kenji, Yamato, Kenji, Takahashi, Fumitaka, Juni, Aya, Saigo, Kaoru
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2008
Assuntos:
RNA
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2367719/
https://ncbi.nlm.nih.gov/pubmed/18267968
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn042
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