Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis.

To generate a collection of conditionally defective poliovirus mutants, clustered charged-to-alanine mutagenesis of the RNA-dependent RNA polymerase 3D was performed. Clusters of charged residues in the polymerase coding region were replaced with alanines by deoxyoligonucleotide-directed mutagenesis...

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Detalles Bibliográficos
Publicado en:J Virol
Autores principales: Diamond, S E, Kirkegaard, K
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 1994
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC236523/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8289389/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.68.2.863-876.1994
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