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Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis.

To generate a collection of conditionally defective poliovirus mutants, clustered charged-to-alanine mutagenesis of the RNA-dependent RNA polymerase 3D was performed. Clusters of charged residues in the polymerase coding region were replaced with alanines by deoxyoligonucleotide-directed mutagenesis...

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Autors principals: Diamond, S E, Kirkegaard, K
Format: Artigo
Idioma:Inglês
Publicat: 1994
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC236523/
https://ncbi.nlm.nih.gov/pubmed/8289389
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