Expression, purification and crystallization of human 5-lipoxygenase-activating protein with leukotriene-biosynthesis inhibitors

The nuclear membrane protein 5-lipoxygenase-activating protein (FLAP) plays an essential role in leukotriene synthesis. Recombinant full-length human FLAP with a C-terminal hexahistidine tag has been expressed and purified from the cytoplasmic membrane of Escherichia coli. Diffraction-quality crysta...

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Detalhes bibliográficos
Main Authors: Xu, Shihua, McKeever, Brian M., Wisniewski, Douglas, Miller, Douglas K., Spencer, Robert H., Chu, Lin, Ujjainwalla, Feroze, Yamin, Ting-Ting, Evans, Jilly F., Becker, Joseph W., Ferguson, Andrew D.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2344111/
https://ncbi.nlm.nih.gov/pubmed/18084092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107055571
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