Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5′-phosphate-dependent aminotransferase

Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5′-phosphate-dependent aminotransferase with a molecular weight of 48 168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified...

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Detalhes bibliográficos
Main Authors: Seetharamappa, Jaldappagari, Oke, Muse, Liu, Huanting, McMahon, Stephen A., Johnson, Kenneth A., Carter, Lester, Dorward, Mark, Zawadzki, Michal, Overton, Ian M., van Niekirk, C. A. Johannes, Graham, Shirley, Botting, Catherine H., Taylor, Garry L., White, Malcolm F., Barton, Geoffrey J., Coote, Peter J., Naismith, James H.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2335000/
https://ncbi.nlm.nih.gov/pubmed/17565195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107019562
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