Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5′-phosphate-dependent aminotransferase

Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5′-phosphate-dependent aminotransferase with a molecular weight of 48 168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified...

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Autors principals: Seetharamappa, Jaldappagari, Oke, Muse, Liu, Huanting, McMahon, Stephen A., Johnson, Kenneth A., Carter, Lester, Dorward, Mark, Zawadzki, Michal, Overton, Ian M., van Niekirk, C. A. Johannes, Graham, Shirley, Botting, Catherine H., Taylor, Garry L., White, Malcolm F., Barton, Geoffrey J., Coote, Peter J., Naismith, James H.
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2007
Matèries:
Crystallization Communications
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2335000/
https://ncbi.nlm.nih.gov/pubmed/17565195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107019562
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