Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5′-phosphate-dependent aminotransferase

Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5′-phosphate-dependent aminotransferase with a molecular weight of 48 168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified...

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Hlavní autoři: Seetharamappa, Jaldappagari, Oke, Muse, Liu, Huanting, McMahon, Stephen A., Johnson, Kenneth A., Carter, Lester, Dorward, Mark, Zawadzki, Michal, Overton, Ian M., van Niekirk, C. A. Johannes, Graham, Shirley, Botting, Catherine H., Taylor, Garry L., White, Malcolm F., Barton, Geoffrey J., Coote, Peter J., Naismith, James H.
Médium: Artigo
Jazyk:Inglês
Vydáno: International Union of Crystallography 2007
Témata:
Crystallization Communications
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2335000/
https://ncbi.nlm.nih.gov/pubmed/17565195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107019562
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