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Structural and Mechanistic Analysis of Trichodiene Synthase using Site-Directed Mutagenesis: Probing the Catalytic Function of Tyrosine-295 and the Asparagine-225/Serine-229/Glutamate-233–Mg(2+)(B) Motif,
Trichodiene synthase from Fusarium sporotrichioides contains two metal ion-binding motifs required for the cyclization of farnesyl diphosphate: the “aspartate-rich” motif D(100)DXX(D/E) that coordinates to Mg(2+)(A) and Mg(2+)(C), and the “NSE/DTE” motif N(225)DXXSXXXE that chelates Mg(2+)(B) (boldf...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2329581/ https://ncbi.nlm.nih.gov/pubmed/17996718 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2007.10.015 |
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