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Role of Arginine-304 in the Diphosphate-Triggered Active Site Closure Mechanism of Trichodiene Synthase

The X-ray crystal structures of R304K trichodiene synthase and its complexes with inorganic pyrophosphate (PP(i)) and aza analogues of the bisabolyl carbocation intermediate are reported. The R304K substitution does not cause large changes in the overall structure in comparison with the wild-type en...

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Detalhes bibliográficos
Main Authors: Vedula, L. Sangeetha, Cane, David E., Christianson, David W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2005
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1386727/
https://ncbi.nlm.nih.gov/pubmed/16171386
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0510476
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