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A buried lysine that titrates with a normal pK(a): Role of conformational flexibility at the protein–water interface as a determinant of pK(a)values

Previously we reported that Lys, Asp, and Glu residues at positions 66 and 92 in staphylococcal nuclease (SNase) titrate with pK(a) values shifted by up to 5 pK(a) units in the direction that promotes the neutral state. In contrast, the internal Lys-38 in SNase titrates with a normal pK(a). The crys...

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Autori principali: Harms, Michael J., Schlessman, Jamie L., Chimenti, Michael S., Sue, Gloria R., Damjanović, Ana, García-Moreno E., Bertrand
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2327280/
https://ncbi.nlm.nih.gov/pubmed/18369193
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.073397708
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