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Large shifts in pK(a) values of lysine residues buried inside a protein
Internal ionizable groups in proteins are relatively rare but they are essential for catalysis and energy transduction. To examine molecular determinants of their unusual and functionally important properties, we engineered 25 variants of staphylococcal nuclease with lysine residues at internal posi...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3069169/ https://ncbi.nlm.nih.gov/pubmed/21389271 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1010750108 |
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