Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residue at positions of codon degeneracy.

We have developed a method for differentiating polioviruses from nonpolio enteroviruses using PCR. A pair of panpoliovirus PCR primers were designed to match intervals encoding amino acid sequences within VP1 that are strongly conserved among polioviruses. The initiating primer hybridizes with codon...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Kilpatrick, D R, Nottay, B, Yang, C F, Yang, S J, Mulders, M N, Holloway, B P, Pallansch, M A, Kew, O M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229447/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8940436/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.12.2990-2996.1996
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