Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residue at positions of codon degeneracy.

We have developed a method for differentiating polioviruses from nonpolio enteroviruses using PCR. A pair of panpoliovirus PCR primers were designed to match intervals encoding amino acid sequences within VP1 that are strongly conserved among polioviruses. The initiating primer hybridizes with codon...

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Detalhes bibliográficos
Main Authors: Kilpatrick, D R, Nottay, B, Yang, C F, Yang, S J, Mulders, M N, Holloway, B P, Pallansch, M A, Kew, O M
Formato: Artigo
Idioma:Inglês
Publicado em: 1996
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC229447/
https://ncbi.nlm.nih.gov/pubmed/8940436
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