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Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residue at positions of codon degeneracy.

We have developed a method for differentiating polioviruses from nonpolio enteroviruses using PCR. A pair of panpoliovirus PCR primers were designed to match intervals encoding amino acid sequences within VP1 that are strongly conserved among polioviruses. The initiating primer hybridizes with codon...

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Udgivet i:J Clin Microbiol
Principais autores: Kilpatrick, D R, Nottay, B, Yang, C F, Yang, S J, Mulders, M N, Holloway, B P, Pallansch, M A, Kew, O M
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229447/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8940436/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.12.2990-2996.1996
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