Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residue at positions of codon degeneracy.

We have developed a method for differentiating polioviruses from nonpolio enteroviruses using PCR. A pair of panpoliovirus PCR primers were designed to match intervals encoding amino acid sequences within VP1 that are strongly conserved among polioviruses. The initiating primer hybridizes with codon...

Täydet tiedot

Bibliografiset tiedot

Julkaisussa:	J Clin Microbiol
Päätekijät:	Kilpatrick, D R, Nottay, B, Yang, C F, Yang, S J, Mulders, M N, Holloway, B P, Pallansch, M A, Kew, O M
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	American Society for Microbiology (ASM) 1996
Aiheet:	Research Article
Linkit:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229447/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8940436/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.12.2990-2996.1996
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