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Type-Specific Detection of Echovirus 30 Isolates Using Degenerate Reverse Transcriptase PCR Primers

Following an approach used to specifically identify polioviruses and enterovirus 71, we have developed reverse transcriptase (RT) PCR primers containing mixed-base residues or deoxyinosine at positions of codon degeneracy. These primers permit specific RT-PCR amplification of echovirus 30 (E30) sequ...

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Bibliografiske detaljer
Main Authors: Kilpatrick, David R., Quay, Jacqueline, Pallansch, Mark A., Oberste, M. Steven
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2001
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC87928/
https://ncbi.nlm.nih.gov/pubmed/11283045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.4.1299-1302.2001
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