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Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis

TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each...

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Detalhes bibliográficos
Main Authors: Jenkins, Amy L., Zhang, Yang, Ealick, Steven E., Begley, Tadhg P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2293645/
https://ncbi.nlm.nih.gov/pubmed/18054064
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bioorg.2007.10.005
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