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Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis
TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2007
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2293645/ https://ncbi.nlm.nih.gov/pubmed/18054064 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bioorg.2007.10.005 |
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