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Motor step size and ATP coupling efficiency of the dsDNA translocase EcoR124I

The Type I restriction-modification enzyme EcoR124I is an archetypical helicase-based dsDNA translocase that moves unidirectionally along the 3′–5′ strand of intact duplex DNA. Using a combination of ensemble and single-molecule measurements, we provide estimates of two physicochemical constants tha...

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Autors principals: Seidel, Ralf, Bloom, Joost GP, Dekker, Cees, Szczelkun, Mark D
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 2008
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2291450/
https://ncbi.nlm.nih.gov/pubmed/18388857
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/emboj.2008.69
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