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Motor step size and ATP coupling efficiency of the dsDNA translocase EcoR124I
The Type I restriction-modification enzyme EcoR124I is an archetypical helicase-based dsDNA translocase that moves unidirectionally along the 3′–5′ strand of intact duplex DNA. Using a combination of ensemble and single-molecule measurements, we provide estimates of two physicochemical constants tha...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2291450/ https://ncbi.nlm.nih.gov/pubmed/18388857 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/emboj.2008.69 |
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