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Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion

Thermally responsive elastin like polypeptides (ELPs) can be used to purify proteins from Escherichia coli culture when proteins are expressed as a fusion with an ELP. Nonchromatographic purification of ELP fusion proteins, termed inverse transition cycling (ITC), exploits the reversible soluble–ins...

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Bibliografski detalji
Glavni autori: Trabbic-Carlson, Kimberly, Liu, Li, Kim, Bumjoon, Chilkoti, Ashutosh
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2004
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2287301/
https://ncbi.nlm.nih.gov/pubmed/15557268
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04931604
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