Fusion order controls expression level and activity of elastin-like polypeptide fusion proteins

We have previously developed a method to purify recombinant proteins, termed inverse transition cycling (ITC) that eliminates the need for column chromatography. ITC exploits the inverse solubility phase transition of an elastin-like polypeptide (ELP) that is fused to a protein of interest. In ITC,...

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Detalhes bibliográficos
Main Authors: Christensen, Trine, Amiram, Miriam, Dagher, Sue, Trabbic-Carlson, Kimberly, Shamji, Mohammed F, Setton, Lori A, Chilkoti, Ashutosh
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2775208/
https://ncbi.nlm.nih.gov/pubmed/19533768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.157
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