High-throughput automated refolding screening of inclusion bodies

One of the main stumbling blocks encountered when attempting to express foreign proteins in Escherichia coli is the occurrence of amorphous aggregates of misfolded proteins, called inclusion bodies (IB). Developing efficient protein native structure recovery procedures based on IB refolding is there...

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Main Authors: Vincentelli, Renaud, Canaan, Stéphane, Campanacci, Valérie, Valencia, Christel, Maurin, Damien, Frassinetti, Frédéric, Scappucini-Calvo, Loréna, Bourne, Yves, Cambillau, Christian, Bignon, Christophe
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2004
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2286565/
https://ncbi.nlm.nih.gov/pubmed/15388864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04806004
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