High-throughput automated refolding screening of inclusion bodies

One of the main stumbling blocks encountered when attempting to express foreign proteins in Escherichia coli is the occurrence of amorphous aggregates of misfolded proteins, called inclusion bodies (IB). Developing efficient protein native structure recovery procedures based on IB refolding is there...

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Detalhes bibliográficos
Main Authors: Vincentelli, Renaud, Canaan, Stéphane, Campanacci, Valérie, Valencia, Christel, Maurin, Damien, Frassinetti, Frédéric, Scappucini-Calvo, Loréna, Bourne, Yves, Cambillau, Christian, Bignon, Christophe
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2004
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2286565/
https://ncbi.nlm.nih.gov/pubmed/15388864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04806004
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