Shear stress-mediated refolding of proteins from aggregates and inclusion bodies

Recombinant protein overexpression of large proteins in bacteria often results in insoluble and misfolded proteins directed to inclusion bodies. We report the application of shear stress in micrometer-wide, thin fluid films to refold boiled hen egg white lysozyme, recombinant hen egg white lysozyme,...

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Veröffentlicht in:Chembiochem
Hauptverfasser: Yuan, Tom Z., Ormonde, Callum F.G., Kudlacek, Stephan T., Kunche, Sameeran, Smith, Joshua N., Brown, William A., Pugliese, Kaitlin M., Olsen, Tivoli J., Iftikhar, Mariam, Raston, Colin L., Weiss, Gregory A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4388321/
https://ncbi.nlm.nih.gov/pubmed/25620679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201402427
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