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Shear stress-mediated refolding of proteins from aggregates and inclusion bodies
Recombinant protein overexpression of large proteins in bacteria often results in insoluble and misfolded proteins directed to inclusion bodies. We report the application of shear stress in micrometer-wide, thin fluid films to refold boiled hen egg white lysozyme, recombinant hen egg white lysozyme,...
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| Publicado no: | Chembiochem |
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| Main Authors: | , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4388321/ https://ncbi.nlm.nih.gov/pubmed/25620679 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201402427 |
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