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Control of aggregation in protein refolding: the temperature-leap tactic.

The kinetics of renaturation of bovine carbonic anhydrase II (CAII) were studied from 4 degrees to 36 degrees, at the relatively high [CAII] of 4 mg/mL. Following dilution to 1 M guanidinium chloride, aggregate formation is very rapid and reduces the formation of active enzyme. The CAII activity yie...

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Bibliografske podrobnosti
Main Authors: Xie, Y., Wetlaufer, D. B.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 1996
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143365/
https://ncbi.nlm.nih.gov/pubmed/8868489
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