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Thermostability of multidomain proteins: Elongation factors EF-Tu from Escherichia coli and Bacillus stearothermophilus and their chimeric forms
Recombinant mesophilic Escherichia coli (Ec) and thermophilic Bacillus stearothermophilus (Bst) elongation factors EF-Tus, their isolated G-domains, and six chimeric EF-Tus composed of domains of either EF-Tu were prepared, and their GDP/GTP binding activities and thermostability were characterized....
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2286523/ https://ncbi.nlm.nih.gov/pubmed/14691225 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.03272504 |
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