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Cloning and Expression of Thermostable α-Amylase Gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis

The structural gene for a thermostable α-amylase from Bacillus stearothermophilus was cloned in plasmids pTB90 and pTB53. It was expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about fivefold more α-amylase (20.9 U/mg of...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Aiba, Shuichi, Kitai, Kazuo, Imanaka, Tadayuki
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1983
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC239519/
https://ncbi.nlm.nih.gov/pubmed/16346413
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