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Cloning and Expression of Thermostable α-Amylase Gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis

The structural gene for a thermostable α-amylase from Bacillus stearothermophilus was cloned in plasmids pTB90 and pTB53. It was expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about fivefold more α-amylase (20.9 U/mg of...

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Détails bibliographiques
Auteurs principaux: Aiba, Shuichi, Kitai, Kazuo, Imanaka, Tadayuki
Format: Artigo
Langue:Inglês
Publié: 1983
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC239519/
https://ncbi.nlm.nih.gov/pubmed/16346413
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